Interactions of p24 proteins characterized by yeast two-hybrid, mutagenesis, and overexpression
Bibliographic record
Abstract
Les abondantes protéines de la famille p24 sont conservées dans l’évolution et circulent dans la voie sécrétoire précoce, mais leurs fonctions cellulaires sont encore mal définies. Ils sont dispensables chez la levure, mais un knock-out de p23 est létal embryonnaire chez la souris. De plus, p23 est impliqué dans la pathogénèse de la maladie d’Alzheimer. Les protéines p24 peuvent former des hétéro-complexes. Dans cette étude, les interactions des p24s sont déterminées par double hybride. Elles sont spécifiques et se font surtout par leur domaine GOLD avec une contribution de leur séquence DOG. Les expériences de mutagénèse révèlent que deux p24s interagissent différemment avec un p24 commun. La co-surexpression de certains p24s dans la levure cause la mort ou l’élargissement des cellules. Je propose que les protéines p24s participent dans le transport des protéines à ancrage GPI impliquées dans l’entretien de la paroi cellulaire et dans les voies du cycle cellulaire.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".