Engineering of bacteriophages displaying affinity tags on its head for biosensor applications
Bibliographic record
Abstract
A method was developed for the introduction of affinity tags onto bacteriophage heads using a phage display technique. It was shown that T4 bacteriophage carrying a biotin-binding polypeptide (Biotin Carboxyl Carrier Protein, BCCP, from acetyl-CoA carboxylase of E. coli) or a Cellulose Binding Module (CBM, of xylanase from Thermotoga maritime) can be effectively immobilized onto various streptavidin- or cellulose-based materials, respectively. Interaction of the bacteriophage with the functionalized surface is strong and almost irreversible. The immobilized phages, termed BCCP-T4 and CBM-T4, retain a high level of infectivity and are capable of capturing 70100% of E. coli cells from suspensions containing 10 to 10 6 cfu/ml in 30 min. The subsequent lysis of captured cells resulted in the release of multiple progeny phages. Enumeration of progeny phages by real-time PCR allowed detection of 1 cfu of E. coli per 5 µl sample within 2 hours.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".