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Record W2188565372 · doi:10.82308/26380

Elucidation of the functional consequences of «NLRP7» mutations

2009· article· en· W2188565372 on OpenAlexaboutno aff
Ebtihaj Bukhari

Bibliographic record

VenueeScholarship@McGill (McGill) · 2009
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicInflammasome and immune disorders
Canadian institutionsnot available
Fundersnot available
KeywordsBiologyGeneticsEvolutionary biologyComputational biology

Abstract

fetched live from OpenAlex

Hydatidiform mole (HM) is an abnormal human pregnancy characterized by the absence of, or abnormal, embryonic development and hydropic degeneration of the chorionic villi. In both Canada and the United States, the incidence of HM is 1 in every 1000 pregnancies. Recently, NLRP7 has been found to be responsible for recurrent hydatidiform moles (RHM) after the identification of various mutations in this gene. To investigate the functional consequences of NLRP7 mutations on IL-1β maturation and secretion, I used site-directed mutagenesis to introduce the various mutations found in patients with RHM into WT-NLRP7. HEK293 cell lines were cotransfected with WT-NLRP7, procaspase-1, and pro-IL-1β, as well as with or without Cardinal (CARD-8.). My results demonstrate that WT-NLRP7 inhibits IL-1β secretion in a dose-dependent manner. Furthermore, I found that Cardinal (CARD8), an important component of other inflammasomes, has no impact on the inhibitory effect of WT-NLRP7. My findings strengthen the idea that NLRP7 may function as a feedback regulator of IL-1β secretion.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.003

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.219
Teacher spread0.208 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2009
Admission routes1
Has abstractyes

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