A Phase I Study with Long-Term Follow-Up of Autologous Stem Cell Transplantation Using Photodynamic Treatment of Marrow Grafts for Relapsed/Refractory Acute Leukemia.
Bibliographic record
Abstract
Abstract Autologous transplantation for acute leukemia is complicated by an elevated risk of leukemia relapse. Effective purging of stem cell grafts along with dose-intensive consolidation may be beneficial to patients who are ineligible for allogeneic transplantation. In pre-clinical studies, the photosensitizing agent benzoporphyrin derivative-monoacid A (BPD-MA) (QLT, Vancouver, Canada) demonstrated the ability to selectively target leukemia cells, generating fluorescence levels 2- to 20-fold higher on these cells than on normal cells. When exposed to light, BPD-MA induced in vitro photodynamic killing that was drug-dose, light-dose and cell-density dependent. This agent eliminated 3 to 6 logs of leukemia cell lines among normal hematopoietic cells as evaluated using in vitro limiting dilution assays and in vivo infusion of such treated grafts in murine recipients. In contrast, BPD-MA used in the same conditions was toxic to only one log of normal hematopoietic progenitors. These impressive results prompted us to perform a clinical Phase I trial to address the feasibility and safety of BPD-MA photodynamic therapy (PDT) for purging of marrow grafts prior to autologous transplantation in patients with high risk acute leukemia. Patients with acute leukemia in second or subsequent complete remission (CR), or with initial refractory disease were eligible. We evaluated 11 patients (6 female) with relapsed (n=10) or refractory (n=1) acute leukemia (acute myeloid leukemia = 10 pts; acute lymphoblastic leukemia = 1 pt) who were ineligible for allogeneic transplantation due to lack of an appropriate donor (8 patients) or age > 55 (3 patients). Median age at transplant was 44 years (range 17–63). All patients were transplanted with marrow grafts harvested immediately before the transplant and exposed to increasing doses of BPD-MA (10, 20 and 25 ng/ml) under a constant light dose (15 joules/cm2). Photodynamic treatment reduced mononuclear cell number by 28 ± 4.9%. Hematopoietic precursors in the graft were decreased by 59–81% (CFU-GM: 10.3 to 4.2x104/kg, p=0.018; BFU-E: 6.0 to 2.1x104/kg, p=0.007; and CFU-GEMM: 2.0 to 0.4x103/kg, p=0.015). Patients received busulphan (16 mg/kg) and cyclophosphamide (150 mg/kg) as myeloablative conditioning followed by infusion of the PDT treated stem cell graft. Median time to neutrophil (>0.5 x 109/l) and platelet engraftment (>20 x 109/l) was 27 days (range 17–68) and 81 days (51–224), respectively. Failure to engraft with neutrophils (>0.5 x 109/l) on day 40 occurred in 2 patients at 25 ng/ml BPD-MA, and maximum tolerated PDT dose was defined as 20 ng/ml BPD-MA. There was no procedure-related mortality, we observed usual rates of infectious complications, and non-hematologic toxicity was not identified. The estimated 5-year overall and disease-free survival is identical at 36 ± 14% with 4 patients alive in CR 41 to 91 months (median, 60 months) after transplantation. These results show that marrow purging with BPD-MA allows hematopoietic engraftment and adds no significant toxicity to the transplant procedure. The long-term follow-up in our study indicates that autologous marrow transplantation with photodynamic purging using BPD-MA is feasible and associated with encouraging rates of disease-free survival for patients with relapsed or refractory acute leukemia.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.003 | 0.001 |
| Meta-epidemiology (narrow) | 0.002 | 0.001 |
| Meta-epidemiology (broad) | 0.002 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.003 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".