Bibliographic record
Abstract
INTRODUCTION: Spermatogonial stem cells (SSCs) form the foundation of fertility throughout the life of the adult male. However, pathways controlling their self-renewal versus differentiation remain poorly understood. It has previously been established that the deubiquinating enzyme Ubiquitin Carboxyl-Terminal Hydrolase 1 (UCH-L1) is specifically expressed in undifferentiated spermatogonia that contain the SSC population [1]. Based on previous observations that UCH-L1 expression decreases with germ-line differentiation, it is hypothesized that UCH-L1 may play a role in SSC maintenance. The overall goal of the project is to elucidate the role of UCH-L1 in an immortalized type A spermatogonia mouse cell line. METHODS: This project uses C18-4 cells [2], an immortalized type A mouse spermatogonia cell line, treated with a specific, reversible, active site directed inhibitor of UCH-L1 to examine the growth characteristics of germ cells in the absence of UCH-L1 activity. Cells were treated with 5µM, 10µM and 20µM of UCH-L1 inhibitor compared to DMSO (vehicle) and no treatment control for 24 and 48 hours. Changes in cell number, viability, proliferation (EdU), and apoptosis (TUNEL) were assessed between treatments. RESULTS: A decreasing trend of cell number and proliferation was observed with increasing UCH-L1 inhibitor concentration. The greatest change in cell number was observed in the 48-hour treatment group with 20µM of UCH-L1 inhibitor (p=0.0659) (Figure 1). There was no change observed in TUNEL or cell viability. DISCUSSION AND CONCLUSIONS: Results analyzed to date show no significant differences between treatments, which is likely due to low replicate number (n=2). However, a trend of decreasing cell number and proliferation was observed with increasing UCH-L1 inhibitor concentration for both 24 hour and 48-hour treatments. Results of these experiments will help determine the appropriate inhibitor treatment to further examine the mechanistic actions of UCH-L1. Furthermore, elucidating this pathway may lead to new strategies to support expansion of SSCs in vitro .
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".