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Adaptive Response with Oxidative Stress from CT Scans and Exercise in Mice

2008· article· en· W2253860275 on OpenAlexafffund
Nghi Phan, Michael De Lisio, Gianni Parise, Doug Boreham

Bibliographic record

VenueThe FASEB Journal · 2008
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicCarcinogens and Genotoxicity Assessment
Canadian institutionsMcMaster University
FundersNatural Sciences and Engineering Research Council of CanadaU.S. Department of Energy
KeywordsOxidative stressIn vivoDNA damageIn vitroOxidative phosphorylationChemistryNuclear medicineEndocrinologyMedicineInternal medicineDNABiologyBiochemistryGenetics

Abstract

fetched live from OpenAlex

We speculated that oxidative stress produced by repeated low dose diagnostic CT scans (10 mGy/scan, 2d/wk, 10 wk) would induce an adaptive response (AR) in C57BL/6 mice. We postulated that additional oxidative stress produced by exercise (1hr, 3d/wk, 10wk could further enhance the AR. We analyzed DNA double strand break (DSB) levels and genomic damage to stem cells via gH2AX foci and micronucleated reticulocytes (MN‐RET) flow cytometric assays, respectively. Following a challenge dose (1 Gy) in vitro, cells from CT mice had a significant increase (14%) in DNA DSBs compared to control and CT/exercised mice (p<0.035). A higher 2 Gy challenge dose showed that there was a significant reduction (16%) in DNA DSBs in CT/exercised mice (p = 0.002). When genomic damage was assessed, mice that had CT scans had significantly higher levels of MN‐RET (46% higher) compared to controls (p < 0.009). Interesting, the CT/exercise mice did not have any detectable increase in MN‐RET levels. After an in vivo 2 Gy challenge dose, control mice and CT mice had similar high levels of induced MN‐RET but there was a significant reduction (14%) in MN‐RET levels in the CT/exercise mice (p< 0.025). This research shows that CT scans can induce an AR in mice; however, this response is significantly modified by exercise. This research was supported by US DOE, Low Dose Research Program (DE‐FG02‐07ER64343) and NSERC.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.009

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0000.001
Bibliometrics0.0010.000
Science and technology studies0.0000.001
Scholarly communication0.0010.000
Open science0.0000.001
Research integrity0.0010.002
Insufficient payload (model declined to judge)0.0030.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.014
GPT teacher head0.235
Teacher spread0.221 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2008
Admission routes2
Has abstractyes

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