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Record W2255288145

SP8 Sequencing Extinct Genomes

2007· article· en· W2255288145 on OpenAlexaff
Hendrik N. Poinar

Bibliographic record

VenuePubMed Central · 2007
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenomics and Phylogenetic Studies
Canadian institutionsMcMaster University
Fundersnot available
KeywordsMetagenomicsAncient DNAGenomeComputational biologyBiologyEvolutionary biologyDNA sequencingPyrosequencingDNAIdentification (biology)Nucleic acidGeneticsGeneEcology
DOInot available

Abstract

fetched live from OpenAlex

Nucleic acids, which hold clues to the evolution of various animal and hominid taxa, are comparatively weak molecules from other cellular debris, and thus evolutionary biologists are in essence time trapped. Fortunately, DNA and protein fragments do exist in fossil remains beyond what theoretical experimentation would suggest. Sequestering of DNA molecules in humic or Maillard-like complexes likely represents a rich source of DNA molecules from the past, which have yet to be tapped. These molecules were impossible to acquire due to the selective nature of the polymerase chain reaction. Recently, however, rapid parallel pyrosequencing techniques, such as those used in metagenomics-based research, which, in theory, allow for the identification of all short nucleotide sequences in a sample in a non-selective approach, have the potential to allow the identification of all nucleic acids in a sample, and thus represent the way forward for ancient DNA. In theory, this new technology will allow the completion of genomes of extinct animals, plants, and microbes. I will discuss the benefits and pitfalls of this metagenomics approach to ancient DNA, highlighting our recent efforts underway to sequence the wooly mammoth genome as well as other fossil remains.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.005
Threshold uncertainty score0.018

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0020.001
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0010.000
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0050.003

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.015
GPT teacher head0.216
Teacher spread0.201 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2007
Admission routes1
Has abstractyes

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