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Analysis of the oligomerization propensity of prohibitin1 (603.1)

2014· article· en· W2257163800 on OpenAlexafffundabout
Gay Pauline Padilla‐Meier, Markus Meier, Jörg Stetefeld, Suresh Mishra

Bibliographic record

VenueThe FASEB Journal · 2014
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMitochondrial Function and Pathology
Canadian institutionsUniversity of Manitoba
FundersManitoba Health Research Council
KeywordsProhibitinBiogenesisBiochemistryMembrane proteinCytosolChemistryInner mitochondrial membraneMitochondrionBiologyMembraneEnzymeGene

Abstract

fetched live from OpenAlex

Prohibitins (PHB) are highly conserved and ubiquitously expressed proteins in eukaryotic cells. The prohibitin family exist in two isoforms PHB1 (also known as BAP32) and PHB2 ( also known as BAP37 and REA). The PHB1 and PHB2 together form large ring shaped complexes in the inner‐mitochondrial membrane, although PHB has also been reported to be localized in the nucleus and the cell membrane. PHB proteins have been implicated in mitochondrial biogenesis as well as scaffold for mitochondrial proteins. Details on the structure and function of PHB1 are still unclear. To better understand the functional mechanisms of PHB1 , we conducted biophysical analysis on recombinant human PHB1 and a truncated form of PHB1 (aa 52‐197). Our results show that PHB1 variants can form oligomers, and that varying salt concentration can modulate the polymerization of PHB1. Furthermore, addition of iron to PHB1 in solution affects the stability of the PHB1 oligomers. These data suggest that PHB1 could have diverse oligomeric forms depending on its local environment Grant Funding Source : Supported by: Manitoba Health Research Council,

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.004

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.013
GPT teacher head0.228
Teacher spread0.215 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2014
Admission routes3
Has abstractyes

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