The role of amiloride‐insensitive sodium transport in alveolar fluid homeostasis (695.2)
Bibliographic record
Abstract
Under both normal and pathological conditions, the lung fluid balance across the alveolar epithelial barrier is mainly regulated by active sodium transporters. Previous work has largely focused on the critical role of ENaC, yet ~60% of alveolar fluid clearance in rats or humans is amiloride insensitive. We decided to explore the role of the amiloride‐insensitive sodium cotransporters SGLT1 (Na + ‐glucose co‐transporter 1) and SNATs (Na + ‐coupled neutral amino acid transporters) in the intact lung. In isolated perfused rat lungs, we determined alveolar fluid clearance (AFC) using a double indicator dilution technique and probed for the role of SGLT1 and SNATs by addition of appropriate cotransporter substrates or inhibitors. While addition of the SNAT substrate L‐alanine did not affect basal AFC, it rescued AFC in lungs pre‐treated with amiloride, and this response was blocked by the SNAT inhibitor HgCl 2. Analysis of bronchoalveolar lavage fluid revealed > 2‐fold of L‐alanine in the epithelial lining fluid compared to plasma level. RT‐PCR identified mRNAs for all 6 SNAT isoforms in whole lung, yet in alveolar epithelial cells, only SNAT 2 and 5 mRNA and protein were detected. In contrast, no functional involvement of SGLT1 in AFC was detected. These results indicate a new role for SNATs in AFC which becomes unmasked when ENaC is inhibited. SNAT2 and SNAT5 may represent novel targets for the resolution of pulmonary edema. Grant Funding Source : Funded by CIHR MOP # 245251
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".