PAF and H2O2 act synergistically to increase endothelial cell oxidant stress
Bibliographic record
Abstract
We have previously shown that alveolar macrophages from septic mice can 1) induce an oxidant stress in pulmonary endothelial cells (PEC) and 2) promote neutrophil (PMN) transendothelial migration. Both of these responses can be blocked by catalase or a PAF receptor antagonist. In the present study, we assessed whether PAF and/or H2O2 can induce an oxidant stress in endothelial cells. PEC were isolated from murine lungs and exposed to different concentrations of H2O2 (25 – 200 μM) and PAF (10‐10 – 10‐7 M) for 1 hr. Subsequently, PEC oxidant stress was assessed using DHR 123 oxidation as an index. H2O2 increased PEC oxidant stress only when used at the highest concentration (200 μM), while PAF did not increase PEC oxidant stress at any of the concentrations tested. When PAF and H2O2 were added simultaneously to PEC there was a potentiation of PEC oxidant stress. Our findings indicate that PAF and H2O2 act synergistically to promote endothelial cell oxidant stress, most likely through the activation of NADPH oxidase.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".