Optical trapping of mesoscopic transparent/metallic particles by spatially structured laser beam and characterization of trapped RBCs
Bibliographic record
Abstract
Optical tweezer (OT) using laser light has enabled the researchers to carry-out fundamental studies and peep into the microscopic world of natural sciences and engineering. In this thesis, multiparticle trapping of metallic nanostructures, photodamage-free isolation of electrically anisotropic rod-shaped live single cells E. coli bacteria and biological cells like RBCs and EAC cells, and then their characterisation using spatially structured and asymmetric (SSA) laser beam have been presented in seven chapters. In Chapter 1 Fundamentals of optical tweezers (OT), radiation pressure, classification of the working regimes based on trapping wavelength and particles size, forces involved in OTs acting on transparent as well as metallic particles, development of OT, need of spatial beam shaping techniques (SLM, CHGs, e-beam lithographic phase-plate, multiple beam interferometry, holography etc.) and their merits and demerits in optical trapping schemes and up-to-date literature review is covered. Factors, like state of polarization of laser beam, aberrations of the optical system and the consequences of excitation of plasmon waves influencing the light-matter interaction in trapping have been discussed. Predominantly, the central role of SSA laser beam in multiparticle trapping has been augmented. In Chapter 2 Generation of spatial beam patterns from fundamental mode TEMoo (also LPo1 ) emitted from red He-Ne laser light (Milles Garriot) using a 1x2 single mode fiber-coupler (SMFC) has been described. Here it has been investigated that due to presence of birefringence as a result of either of fibre-core asymmetry or a stress-induced waveguide cause the generation of LP 1 modes. Further, spiral pattern of unit charge (sometimes more than one) obtained by interfering LPo 1 and LP 1 modes at beam-splitter is demonstrated for real-time observation of minuscule power-fluctuations of a laser source (2mW, 532nm). In Chapter 3 Multiparticle trapping and assembling of polystyrene beads using SSA laser beam emitted directly from the cavity of diode pumped solid state (Nd 3+ : YVO4 ) laser source (Laser Glow, Canada) operating at 532nm is described. It has been analysed that distributed intensity gradients of SSA beam has generated relatively larger focused spot size than pure TEMoomode, smearing out the centrally confined power which in-turn effected weaker axial scattering force. SSA laser beam (p — polarization) driven OT has achieved in real-time photodamage-free multiple trapping of anisotropic rod-shaped livemicroorganisms. Chapter 4 In-situ clustering of unfunctionalized large diameter Au-NP (150nm and 254nm) by SSA laser beam near plasmon excitation wavelength window has been demonstrated. This
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".