Sensitive detection of bisphenol A based on a ratiometric electrochemical aptasensor
Bibliographic record
Abstract
A new ratiometric electrochemical aptasensor has been developed for highly sensitive and selective detection of bisphenol A (BPA). The double-stranded DNA (dsDNA), consisting of the BPA aptamer (DNA1) and methylene blue (MB)-labeled complementary DNA (cDNA), was immobilized on a gold nanoparticle (AuNP) modified glassy carbon (GC) electrode. In the presence of BPA, the specific BPA–aptamer interaction drove the release of the MB-labeled cDNA from the electrode surface. As a result, the oxidation peak current of MB (IMB) decreased and that of BPA (IBPA) increased. The peak current ratio (IBPA/IMB) of BPA and MB was linear with the concentration of BPA in the range from 1 to 100 pmol/L with a detection limit of 0.6 pmol/L. The detection limit is much lower than that obtained by most of the reported electrochemical methods. On the other hand, the developed aptasensor possesses good selectivity, reproducibility, and stability, and the related sensing structure is very simple, showing promising practical applications in BPA assays.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.002 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.002 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.002 | 0.001 |
| Research integrity | 0.002 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".