MS4A8B oligomerizes with CD20 (MS4A1) and modulates B cell receptor‐stimulated calcium entry
Bibliographic record
Abstract
The MS4A gene family comprises over 20 distinct murine and human genes expressed in hematopoeitic and other tissues. The founding member of this family, CD20 (MS4A1), is highly expressed in B lymphocytes and functions in store‐operated calcium influx activated by B cell receptor crosslinking (Li, H. et al J. Biol. Chem. (2003) 278:42427–34). Immune responses in CD20‐deficient mice appear to be normal, highlighting the possibility of compensating function from other members of the same family. In this study we examined the expression and function of a new member of the human MS4A family, MS4A8B, which was reported to be transcribed in B cell lines but not in cell lines from other hematopoeitic lineages. Using a GFP‐tagged construct expressed in the BJAB human B cell line, we found that MS4A8B co‐localised with CD20 at the plasma membrane and co‐immunoprecipitated with CD20 from detergent lysates. Like CD20, MS4A8B GFP co‐capped with the BCR in anti‐IgM stimulated cells and remained at the plasma membrane following internalization of the BCR. Over‐expression of MS4A8B in a human B cell line enhanced calcium influx following BCR stimulation. The expression of endogenous MS4A8B and its association with CD20 in primary B cells were confirmed using a new polyclonal anti‐MS4A8B antibody. Interestingly, expression of MS4A8B (but not CD20) was rapidly down‐regulated after BCR stimulation, suggesting a differential role for MS4A8B in modulating BCR‐activated calcium signalling. Funded by the Canadian Institutes of Health Research (CIHR).
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".