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Record W2274912893 · doi:10.3389/fmicb.2016.00073

Back to the Future of Soil Metagenomics

2016· article· en· W2274912893 on OpenAlexaff
Joseph Nesme, Wafa Achouak, Spiros N. Agathos, Mark Bailey, Petr Baldrián, Dominique Brunel, Åsa Frostegård, Thierry Heulin, Janet Jansson, Édouard Jurkevitch, Kristiina Kruus, George A. Kowalchuk, Antonio Lagares, Hilary Lappin‐Scott, Philippe Lemanceau, Denis Le Paslier, Ines Mandic‐Mulec, J. Colin Murrell, David D. Myrold, Renaud Nalin, P. Nannipieri, Josh D. Neufeld, Fergal O’Gara, John Jacob Parnell, Alfred Pühler, Victor Satler Pylro, Juan L. Ramos, Luiz Fernando Würdig Roesch, Michael Schloter, Christa Schleper, Alexander Sczyrba, Angela Sessitsch, Sara Sjöling, Jan Sørensen, Søren J. Sørensen, Christoph C. Tebbe, Edward Topp, George Tsiamis, Jan Dirk van Elsas, Geertje van Keulen, Franco Widmer, Michael Wagner, Tong Zhang, Xiaojun Zhang, Liping Zhao, Yong‐Guan Zhu, Timothy M. Vogel, Pascal Simonet

Bibliographic record

VenueFrontiers in Microbiology · 2016
Typearticle
Languageen
FieldEnvironmental Science
TopicMicrobial Community Ecology and Physiology
Canadian institutionsAgriculture and Agri-Food CanadaWestern UniversityUniversity of Waterloo
FundersNatural Environment Research CouncilInstitut National de la Recherche AgronomiqueCentre National de la Recherche ScientifiqueMinistère de l'Education Nationale, de l'Enseignement Supérieur et de la RechercheMinistère de l'Education Nationale, de l'Enseignement Superieur et de la RechercheSight Research UKAgence Nationale de la RechercheNational Science Foundation
KeywordsMetagenomicsBiologyFront (military)EcologyComputational biologyGeologyOceanographyGenetics

Abstract

fetched live from OpenAlex

Direct extraction and characterization of microbial community DNA through PCR amplicon surveys and metagenomics has revolutionized the study of environmental microbiology and microbial ecology. In particular, metagenomic analysis of nucleic acids provides direct access to the genomes of the "uncultivated majority." Accelerated by advances in sequencing technology, microbiologists have discovered more novel phyla, classes, genera, and genes from microorganisms in the first decade and a half of the twenty-first century than since these "many very little living animalcules" were first discovered by van Leeuwenhoek (Table 1). The unsurpassed diversity of soils promises continued exploration of a range of industrial, agricultural, and environmental functions. The ability to explore soil microbial communities with increasing capacity offers the highest promise for answering many outstanding who, what, where, when, why, and with whom questions such as: Which microorganisms are linked to which soil habitats? How do microbial abundances change with changing edaphic conditions? How do microbial assemblages interact and influence one another synergistically or antagonistically? What is the full extent of soil microbial diversity, both functionally and phylogenetically? What are the dynamics of microbial communities in space and time? How sensitive are microbial communities to a changing climate? What is the role of horizontal gene transfer in the stability of microbial communities? Do highly diverse microbial communities confer resistance and resilience in soils?

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.015
metaresearch head score (Gemma)0.013
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Theoretical or conceptual · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.015
Threshold uncertainty score0.079

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0150.013
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0020.001
Bibliometrics0.0020.001
Science and technology studies0.0010.007
Scholarly communication0.0070.020
Open science0.0020.004
Research integrity0.0070.013
Insufficient payload (model declined to judge)0.0080.003

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.005
GPT teacher head0.192
Teacher spread0.187 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designTheoretical or conceptual
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations146
Published2016
Admission routes1
Has abstractyes

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