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Record W2279301141 · doi:10.82308/24817

Tripartite «trans»-splicing of the L1.LtrB group ll intron

2010· article· en· W2279301141 on OpenAlexfundno aff
Christine Ritlop

Bibliographic record

VenueeScholarship@McGill (McGill) · 2010
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicRNA Research and Splicing
Canadian institutionsnot available
FundersNatural Sciences and Engineering Research Council of Canada
KeywordsIntronSpliceosomeGroup II intronRNA splicingBiologyMinor spliceosomeExonGeneticsGroup I catalytic intronRibozymeMolecular biologyRNAGene

Abstract

fetched live from OpenAlex

Les introns de groupe II sont des introns autocatalytiques qui s'épissent des transcrits de pré-mARN, et liguent concurremment les exons flanquant. D'un point de vue évolutif, ils sont considérés comme les ancêtres des introns nucléaires eucaryotes et de leur appareil d'épissage, le spliceosome. On présume que le fractionnement d'un intron de groupe II ancestral a donné naissance aux cinq petits ARN nucléaires (snRNAs - U1, U2, U4, U5, U6) qui constituent le noyau catalytique du spliceosome. Cette hypothèse est supportée par la découverte d'introns de groupe II fragmentés qui s'épissent en trans dans certaines organelles. Nous avons soumis l'intron de groupe II Ll.LtrB de la bactérie Gram-positive Lactococcus lactis à un essai génétique basé sur le transposon EZ-Tn5, suivi par une analyse de conjugaison, afin d'isoler des introns tripartites capable de s'épisser en trans. En démontrant les premières variantes tripartites de Ll.LtrB in vivo, nous fournissons un support expérimental pour le lien évolutif entre les introns de groupe II et les snRNAs du spliceosome eucaryote.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.006

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.010
GPT teacher head0.236
Teacher spread0.226 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2010
Admission routes1
Has abstractyes

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