N‐cadherin and actin are present at desmosomes in the seminiferous epithelium of the adult testis
Bibliographic record
Abstract
Desmosomes in the seminiferous epithelium of the adult mammalian testis are not as well defined morphologically as those in most other epithelia. Moreover, the plasma membrane related plaques in Sertoli cells are linked to vimentin filaments, not to keratin filaments. Previous studies have indicated that these desmosomes contain classical actin‐related cadherins, thereby raising the interesting possibility that these junctions may have characteristics both of actin and of intermediate filament based junctions. To explore this possibility, we probed the seminiferous epithelium of adult male Spraque Dawly rats for N‐cadherin, actin, and plectin. In fixed frozen sections, N‐cadherin labeled sites at the base of the epithelium that only partly appeared to colocalized with the intensely labeled actin in ectoplasmic specializations. In fragmented epithelium, the actin probe reacted with plectin positive desmosomes. At the ultrastructural level, the probe for N‐cadherin clearly labeled desmosomes. Significantly, an antibody to actin also reacted above background with desmosomes and was localized close to the cytoplasmic face of the membrane related plaques. Our results are consistent with the conclusion that desmosomes in the seminiferous epithelium have characteristics both of actin and of intermediate filament based attachment junctions. Grant Funding Source NSERC
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".