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Effect of Multiparity on Vascular Compliance and Collagen Content

2009· article· en· W2291890741 on OpenAlexaff
Huda E. Tawfik, Mohammad Ali, Richard Schulz, Susan Kaufman

Bibliographic record

VenueThe FASEB Journal · 2009
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicProtease and Inhibitor Mechanisms
Canadian institutionsUniversity of Alberta
Fundersnot available
KeywordsPeroxynitriteHydroxyprolineChemistryAortaInternal medicineAnatomyMatrix metalloproteinaseMasson's trichrome stainEndocrinologyMedicineBiochemistryImmunohistochemistrySuperoxideEnzyme

Abstract

fetched live from OpenAlex

We have shown that vascular compliance is decreased in multiparous rats. When the balance of collagen synthesis/breakdown changes, the functional characteristics of the vessels are altered. Collagen degradation is regulated by matrix metalloproteinases (MMPs). We have previously shown that multiparity increases oxidative stress and peroxynitrite formation. Peroxynitrite can change MMP levels in vascular tissues and thus alter collagen content. We examined the effect of multiparity on collagen content of aorta and mesenteric arteries by Trichrome Masson staining of paraffin imbedded vascular sections, by total vascular collagen and by hydroxyproline. MMP2 activity in vascular tissues was measured by gelatin zymography. Functional changes in vascular compliance were assessed by pressure myography. Data demonstrate that multiparity is associated with decreased MMP2 activity in aorta (870±229 vs 4797±1116) and mesenteric arteries (544±113 vs 2294±777). This was accompanied by increased total collagen (aorta: 536±37 vs 259±32; mesentery: 426±24 vs 206±12) and hydroxyproline (aorta: 149.5±3 vs 64±7; mesentery: 142.8±12.8 vs 74.0±11). Vascular compliance was decreased in vessels from multiparous rats. These data suggest that multiparity decreases vascular compliance via mechanisms involving MMP‐regulated collagen degradation which is downstream of the peroxynitrite signalling pathway.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.010

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0010.000
Science and technology studies0.0000.001
Scholarly communication0.0000.001
Open science0.0000.001
Research integrity0.0010.002
Insufficient payload (model declined to judge)0.0030.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.023
GPT teacher head0.263
Teacher spread0.239 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2009
Admission routes1
Has abstractyes

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