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Record W2293806425 · doi:10.5376/mpb.cn.2011.09.0102

棉花硝酸盐高亲和转运蛋白基因NRT2片段克隆及RNA干扰表达载体构建

2011· article· zh· W2293806425 on OpenAlexvenueno aff
郑丽玲

Bibliographic record

Venue分子植物育种 · 2011
Typearticle
Languagezh
FieldAgricultural and Biological Sciences
TopicResearch in Cotton Cultivation
Canadian institutionsnot available
Fundersnot available
KeywordsComputer science

Abstract

fetched live from OpenAlex

以低氮(0.2 mmol/L)胁迫4 h的氮高效棉花品种新陆早12号为材料,提取棉花总RNA,根据蓖麻和杨树的 NRT 2 基因保守序列设计含酶切位点的特异引物,用RT-PCR技术获得350 bp的片段。将此片段以正向和反向插入到中间载体pUCM-T的GUS内含子序列两端。将正向片段-GUS-反向片段插入pPZP35S,通过酶切和PCR鉴定,成功构建RNAi载体,并成功转入农杆菌GV3101中,为研究棉花硝酸盐高亲和转运蛋基因 NRT 2 的功能奠定一定的基础。

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesInsufficient payload (model declined to judge)
Consensus categoriesInsufficient payload (model declined to judge)
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.731
Threshold uncertainty score0.997

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0130.003

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.227
GPT teacher head0.300
Teacher spread0.072 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; both teacher heads agree on what is shown here.

Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2011
Admission routes1
Has abstractyes

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