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Record W2294819910 · doi:10.5376/mpb.cn.2011.09.0082

甘薯IbSPF1基因植物表达载体的构建

2011· article· zh· W2294819910 on OpenAlexvenueno aff
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Bibliographic record

Venue分子植物育种 · 2011
Typearticle
Languagezh
FieldAgricultural and Biological Sciences
TopicPolysaccharides and Plant Cell Walls
Canadian institutionsnot available
Fundersnot available
KeywordsComputer science

Abstract

fetched live from OpenAlex

为了获得具有多种抗逆特性的转基因甘薯材料,本文构建了由甘薯过氧化物胁迫诱导型启动子SWPA2和植物组成型启动子CaMV 35S分别控制、且含有甘薯SP8的结合蛋白基因( IbSPF1 )的过表达植物载体。IbSPF1是植物WRKY转录因子家族的成员之一。由于其参与植物多种生物和非生物胁迫的反应以及许多生理过程,WRKY类家族越来越成为人们研究的热点。本研究利用RT-PCR方法从甘薯茎叶组织中扩增出编码 IbSPF1 的完整开放阅读框,与已发表的 IbSPF1 相比,从翻译起始点开始第1 009位点的胸腺嘧啶转变为胞嘧啶,从而使翻译产物由色氨酸转变为脯氨酸。本研究进一步构建了由不同启动子控制、携带 IbSPF1 基因的超表达植物载体pCAMBIA2300,并转入到根癌农杆菌菌株EHA105中,为未来甘薯抗逆转基因打下基础。

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.010
Threshold uncertainty score0.019

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0010.001
Scholarly communication0.0020.003
Open science0.0010.001
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0050.002

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.063
GPT teacher head0.198
Teacher spread0.135 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

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Citations0
Published2011
Admission routes1
Has abstractyes

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