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Record W2296550515 · doi:10.1038/ng.3526

The spotted gar genome illuminates vertebrate evolution and facilitates human-teleost comparisons

2016· article· en· W2296550515 on OpenAlexaff
Ingo Braasch, Andrew R. Gehrke, Jeramiah J. Smith, Kazuhiko Kawasaki, Tereza Manousaki, Jérémy Pasquier, Angel Amores, Thomas Desvignes, Peter Batzel, Julian Catchen, Aaron M. Berlin, Michael S. Campbell, Daniel Barrell, Kyle J. Martin, John F. Mulley, Vydianathan Ravi, Alison Lee, Tetsuya Nakamura, Domitille Chalopin, Shaohua Fan, Dustin J. Wcisel, Cristian Cañestro, Jason Sydes, Felix E.G. Beaudry, Yi Sun, Jana Hertel, Michael J. Beam, Mario Fasold, Mikio Ishiyama, Jeremy Johnson, Marcia Lara, John Letaw, Gary W. Litman, Ronda T. Litman, Masato Mikami, Tatsuya Ota, Nil Ratan Saha, Louise Williams, Peter F. Stadler, Han Wang, John S. Taylor, Quenton Fontenot, Allyse Ferrara, Stephen M. J. Searle, Bronwen Aken, Mark Yandell, Igor Schneider, Jeffrey A. Yoder, Jean‐Nicolas Volff, Axel Meyer, Chris T. Amemiya, Byrappa Venkatesh, Yann Guiguen, Julien Bobe, Neil H. Shubin, Federica Di Palma, Jessica Alföldi, Kerstin Lindblad‐Toh, John H. Postlethwait

Bibliographic record

VenueNature Genetics · 2016
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicCancer-related molecular mechanisms research
Canadian institutionsUniversity of Victoria
FundersEunice Kennedy Shriver National Institute of Child Health and Human DevelopmentNational Human Genome Research InstituteNational Institute of General Medical SciencesBiomedical Research CouncilConselho Nacional de Desenvolvimento Científico e TecnológicoJapan Society for the Promotion of ScienceDirectorate for Biological SciencesVolkswagen FoundationNational Institutes of HealthNational Center for Research ResourcesNational Institute of Allergy and Infectious DiseasesAgence Nationale de la RechercheMinistry of National Education and Religious AffairsMinisterio de Ciencia e InnovaciónGeneralitat de CatalunyaNational Natural Science Foundation of ChinaAgency for Science, Technology and ResearchBroad InstituteEuropean Molecular Biology LaboratoryUniversity of OregonBiotechnology and Biological Sciences Research CouncilWellcome TrustAlexander von Humboldt-StiftungNational Key Research and Development Program of ChinaBrinson FoundationUniversity of ChicagoHarvard UniversityAgència de Gestió d'Ajuts Universitaris i de RecercaNational Science Foundation
KeywordsBiologyGenomeSubfunctionalizationVertebrateHox geneEvolutionary biologyLineage (genetic)Gene duplicationGeneGeneticsGenome evolutionHuman genomeComparative genomicsGene familyGenomicsGene expression

Abstract

fetched live from OpenAlex

Ingo Braasch, John Postlethwait and colleagues report the genome of the spotted gar (Lepisosteus oculatus), whose lineage diverged from teleosts before genome duplication. Their data provide insights into the evolution of genes involved in immunity, mineralization and development and facilitate the comparison of cis-regulatory elements between teleosts and humans. To connect human biology to fish biomedical models, we sequenced the genome of spotted gar (Lepisosteus oculatus), whose lineage diverged from teleosts before teleost genome duplication (TGD). The slowly evolving gar genome has conserved in content and size many entire chromosomes from bony vertebrate ancestors. Gar bridges teleosts to tetrapods by illuminating the evolution of immunity, mineralization and development (mediated, for example, by Hox, ParaHox and microRNA genes). Numerous conserved noncoding elements (CNEs; often cis regulatory) undetectable in direct human-teleost comparisons become apparent using gar: functional studies uncovered conserved roles for such cryptic CNEs, facilitating annotation of sequences identified in human genome-wide association studies. Transcriptomic analyses showed that the sums of expression domains and expression levels for duplicated teleost genes often approximate the patterns and levels of expression for gar genes, consistent with subfunctionalization. The gar genome provides a resource for understanding evolution after genome duplication, the origin of vertebrate genomes and the function of human regulatory sequences.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.011

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.001
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0030.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.264
Teacher spread0.257 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations671
Published2016
Admission routes1
Has abstractyes

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