IN VITRO 3D СИСТЕМЫ КАК АЛЬТЕРНАТИВА ОПУХОЛЕВЫМ МОДЕЛЯМ IN VIVO
Bibliographic record
Abstract
New models of tumor growth, as an alternative to animal xenografts, play an important role in pre-clinical studies. Substrate-independent 3D multicellular tumor structures are the adequate models of malignant node formation at the avascular growth phase. We performed a comparative analysis of proliferative parameters for breast cancer cells MCF-7 in 2D and 3D culture during prolonged incubation under the condition of starvation. We discovered that survival and resistance to the adverse factors in 3D culture were higher than in 2D culture under the condition of substrates deficiency. Therefore, 3D culture is an adequate model to study cytotoxic and antiproliferative signals in evaluation of substances with antitumor properties. References 1. Mueller-Klieser W. / Crit. Rev. Oncol./Hematol. 2000.V. 36. P.123–139. 2. Mazzoleni G., Di Lorenzo D., Steimberg N. / Genes Nutr. 2009. V. 4. P.13-22. 3. Kunz-Schughart L.A., Freyer J.P., Hofstaedter F., Ebner R. / J. Biomol. Screen. 2004. V.9. P.273–285 4. Fracasso G., Colombatti M. / Crit. Rev. Oncol./Hematol. 2000. V.36. P.159–178. 5. Madsen S.J, Sun C.H., Tromberg B.J., Cristini V., De Magalhaes N., Hirschberg H. / Lasers Surg. Med. 2006. V.38. P.555–564. 6. Ostrovska L., Garmanchuk L. / Bull. Kiev Nat. Univ. Taras Shevchenko . Ser. Biol. 2013. V.2.No. 64. P.53-57. 7. Breast cancer: prevention and control /World Health Organization. 8. Ivascu A., Kubbies M. / Int. J. Oncol. 2007. V.31. P.1403-1413 9. Kim J., Stein R., O'Hare M. / Breast Canc. Res. Treat. 2004. V.85. P.281–291. 10. Kelm J.M., Timmins N.E., Brown C.J., Fussenegger M., Nielsen L.K. / Biotechnol. Bioeng. 2003 V.83. No. 2. P.173-80. 11. Garmanchuk L.V., Perepelytsina O.M., Sidorenko M.V., Ostapchenko L.I. / Cytol. Gen. 2009. V.43. No.5. P.305-309 12. Arundel C.M., Leith J.T. / Int. J. Radiat. Oncol. Biol. Phys. 1986. V.12. No.1. P.59-67. 13. Mosmann T. / J. Immunol. Methods. 1983. V.65. No.1-2. P. 55-63. 14. Nicoletti I. Migliorati G., Pagliacci M.C. / J. Immunol. Methods. 1991. V.139. No. 2. P.271-80.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.001 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.006 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".