P2Y1 receptor‐mediated potentiation of XII motoneuron (MN) inspiratory motor output in neonatal rat
Bibliographic record
Abstract
Inspiratory rhythm generating networks in the preBötzinger Complex (preBötC) are ~100‐fold more sensitive to ATP than XII MNs that control airway patency. This is believed to reflect in part that the effects of ATP in the preBötC are primarily mediated by P2Y 1 Rs while P2XRs receptors dominate the actions of ATP in XII MNs. However, the effects of P2Y 1 R signaling on XII motor output and MN properties have not been examined. To address this deficit, we applied nerve and whole‐cell recording techniques to rhythmic medullary slices of neonatal rat. Local application of MRS2365 (P2Y 1 R agonist) to the XII nucleus of rhythmic slices increased tonic discharge and evoked 22±2 (0.1 mM) and 33±4% (1 mM, n=16) increases in inspiratory burst amplitude. MRS2365 also increased the frequency and amplitude of spontaneous PSCs in non‐inspiratory and inspiratory MNs alike. In inspiratory MNs, it also increased the charge transferred per inspiratory cycle (12±6%, n=5). The increase in synaptic activity was superimposed on a small, slow, inward current that was similar in all XII MNs, averaging 27±7 pA (1 mM, n=10). Following block action potentials with TTX, MRS2365 had no effect on membrane current or resistance. These data suggest that P2Y 1 Rs do not directly affect XII MN membrane properties, but potentiate inspiratory XII nerve output by specifically modulating synaptic function. Supported by CIHR, NSERC, CIHR‐MFN, AHFMR, CFI, ASRA.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".