RGS13 restricts G‐protein‐coupled receptor‐induced responses in mast cells
Bibliographic record
Abstract
Mast cell degranulation and release of vasoactive mediators and cytokines promotes allergic reactions and innate immunity. Regulator of G protein signaling (RGS) proteins attenuate G‐protein‐coupled receptor (GPCR)‐evoked signaling pathways by binding heterotrimeric G protein alpha subunits and acting as GTPase accelerating proteins (GAPs). Several RGS proteins were expressed in mast cells including RGS13. We found previously that RGS13 suppressed IgE‐mediated degranulation of mouse bone marrow‐derived mast cells (BMMCs) and anaphylaxis (Bansal et al Nat Immunol in press). To determine how RGS13 affected GPCR‐mediated signal transduction in human mast cells, we examine responses of the human mastocytoma cell line (HMC‐1) overexpressing RGS13 or expressing an RGS13‐specific short, hairpin siRNA (shRNA) to GPCR stimulation. Compared to control cells, HMC‐1 cells with reduced RGS13 amounts exhibited heightened Ca2+ influx in response to adenosine, C5a, sphingosine‐1‐phosphate (S‐1P), and CXCL12. Chemotaxis and cytokine (interleukin 8, IL‐8) secretion were increased in shRGS13‐HMC‐1 cells while overexpression of RGS13 in HMC‐1 cells inhibited CXCL12‐induced Ca2+ mobilization and chemotaxis. These results suggest that RGS13 may exert control over GPCR‐evoked biological responses in human mast cells.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".