Distinctive roles of neutrophil LSP1 and endothelial cell LSP1 during neutrophil recruitment (173.29)
Bibliographic record
Abstract
Abstract Leukocyte-specific protein 1 (LSP1) is an intracellular, F-actin- and Ca2+-binding, phosphoprotein expressed in leukocytes and endothelial cells (ECs). Using intravital microscopy and time-lapse video photography, we studied the role of neutrophil LSP1 (N-LSP1) and endothelial cell LSP1 (EC-LSP1) during neutrophil recruitment in the post-capillary venules in mice. The wild-type control, LSP1-deficient (Lsp1−/−) and their chimeric mice were used in this study. Neutrophil recruitment was induced by addition of chemokine MIP-2-containing gel to the observed cremasteric venule. Neutrophil intraluminal crawling, transendothelial migration, and chemotaxis in tissue were determined. We found that both N-LSP1 and EC-LSP1 were involved in neutrophil intraluminal crawling and neutrophil transendothelial migration. After neutrophil transmigration, only N-LSP1 was found to be important for neutrophil migration in the tissue, whereas EC-LSP1, but not N-LSP1, was essential for the directional neutrophil chemotaxis. Although N-LSP1 did not play an important role in neutrophil directional chemotaxis, both neutrophil migration and directional chemotaxis were dependent on the function of p38 mitogen-activated protein kinase, an upstream signaling molecule for N-LSP1, in the emigrated neutrophils in the tissue. Our results suggest that N-LSP1 and EC-LSP1 are both important for neutrophil recruitment but play distinctive roles in different steps of neutrophil recruitment in vivo.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".