Changes in cultured dermal fibroblasts during early passages across five wild bird species
Bibliographic record
Abstract
With the advent of the usage of primary fibroblasts in comparative and evolutionary biology, it is important for researchers to know the extent to which cells might be altered during the culturing process and how much species might differ in response to cell culture. We compared early changes in cell size and lipid composition of primary dermal fibroblasts grown at physiologically relevant oxygen concentrations (5% O2) from wild-caught species of birds. Fibroblasts from American Robins (Turdus migratorius L., 1766) and Bobwhite Quails (Colinus virginianus (L., 1758)) increased in size early in the culture process and cells from all five species of wild-caught birds exhibited changes in lipid-class composition. The two most common phospholipids, phosphatidylcholine and phosphatidylethanolamine, increased in concentration in all species between early passages and later passages of fibroblasts. Some less abundant lipid species, such as cardiolipin and sphingomyelin, exhibited similar concentrations in all three passages that we measured. Other lipid classes, such as cholesterol, increased in some species in later passages and decreased in others. Although results may vary with cell-culture conditions, this study points to a need for researchers comparing multiple species to take precautions when using cell culture, such as experimenting on the earliest possible passage of cells.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".