Investigating Water Interactions with Collagen Using<sup>2</sup>H Multiple Quantum Filtered NMR Spectroscopy To Provide Insights into the Source of Double Quantum Filtered Signal in Tissue
Bibliographic record
Abstract
In an effort to provide insight into the molecular origins of the (2)H double quantum filtered (DQF) NMR signal observed in connective tissue, specifically spinal disc tissue, (2)H multiple quantum filtered (MQF) NMR spectroscopy is used to study the structure and dynamics of D2O in collagen as a function of hydration. Residual quadrupolar coupling constants are measured and decrease from 3500 to 20 Hz while T2 relaxation times increase from 0.65 to 20 ms as hydration increases. Analysis of the data indicates that the quadrupolar coupling and T2 relaxation arises when water molecules spend time in restricted environments. The residual quadrupolar coupling is influenced almost exclusively by the most restricted water sites, the clefts of the triple helices not exposed on the surface of the fibrils, while the T2 relaxation has secondary contributions from less restricted water environments. The magnitudes of the measured values are consistent with results from DQF NMR studies of spinal disc tissue, supporting the assertion that water binding to collagen is a major contributor to the DQF NMR signal observed in spinal disc tissue.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".