Giardia lamblia: Distribuição de Microtúbulos no citoesqueleto de trofozoítos e cistos utilizando taxóide fluorescente
Bibliographic record
Abstract
G. lamblia is a protozoan parasite of the small intestine and the etiologic agent ofgiardiosis, which presents a complex cytoskeleton composed of microtubules. Inthis study, the microtubule cytoskeleton was labeled in trophozoites and cysts ofG. lamblia, using a fluorescent taxoid (FLUTAX-2), a derivative of Taxol, whichbinds to ??-tubulin dimer polymerized. In addition, the effect of metronidazole onthe cytoskeleton of the parasite was also evaluated. FLUTAX-2 was able to labelthe microtubules of trophozoites and cysts, allowing the observation of cytoskeletalstructures, such as flagella, funis, adhesive disk, and median body. Moreover,FLUTAX-2 labeled the trophozoites from several different cultivation times,revealing the 48 hours as the best incubation period. The incubation of parasites inpresence of metronidazole did not show significant alteration on the microtubulelabeling by FLUTAX-2. We have shown the FLUTAX-2 labeling in other protozoa,Trichomonas vaginalis, T. gallinae and Tritrichomonas foetus, and this is the firstreport revealing the binding profile in G. lamblia. Our results contribute to theknowledge of biological and morphological features of this intestinal pathogen. Furthermore, our data showed no modification of microtubule profile labeling inpresence of metronidazole. Finally, a new perspective for giardiosis diagnosticis suggested, since trophozoites and cysts of G. lamblia were labeled with FLUTAX-2.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".