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Record W2319551532 · doi:10.5740/jaoacint.12-174

Analysis of Fumagillin in Honey by LC-MS/MS

2013· article· en· W2319551532 on OpenAlexaffabout
Jasna Dmitrovic, David A. Durden

Bibliographic record

VenueJournal of AOAC International · 2013
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicCoccidia and coccidiosis research
Canadian institutionsCanadian Food Inspection Agency
Fundersnot available
KeywordsFumagillinFormic acidChromatographyAmmonium formateTriple quadrupole mass spectrometerElectrospray ionizationChemistryMass spectrometryAdulterantSelected reaction monitoringTandem mass spectrometryBiology

Abstract

fetched live from OpenAlex

Abstract An LC/MS/MS method has been optimized and validated for the quantification and confirmation of fumagillin in honey. Fumagillin is isolated and concentrated by RP polymeric SPE. Final extracts are analyzed using electrospray ionization LC/MS/MS in positive mode with a formic acid and ammonium formate–acetonitrile mobile phase. Method uncertainty was determined using two analytical systems from different manufacturers. The validation of this LC/MS/MS method for the detection of fumagillin demonstrated overall recovery of 99.3%, precision of 5.9%, expanded uncertainty of 13.2%, and LOD of 0.368 μg/kg using an Agilent 6410 triple quadrupole system. Robustness testing showed that the method is robust against small changes in the method parameters. The method has been successfully applied to test 30 honey samples received as part of the Canadian Food Inspection Agency inspection program for the presence of fumagillin. It also was externally evaluated through proficiency testing and found to be fit-for-purpose.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesInsufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.493
Threshold uncertainty score0.994

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0070.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.014
GPT teacher head0.258
Teacher spread0.245 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations10
Published2013
Admission routes2
Has abstractyes

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