Abstract 2724: Intravesical treatment with Vesicular Stomatitis virus decreases the size of bladder tumors without adversely affecting the health and well-being of immune-competent mice
Bibliographic record
Abstract
Abstract Recent studies have shown that wild-type Vesicular Stomatitis virus (VSV) and the Δ51M recombinant IFN-inducing mutant of wild-type VSV (AV3) have powerful oncolytic activity against bladder cancer cells in an immune-compromised bladder cancer model. The objective of the current study was to determine the safety and efficacy of the wild-type VSV and AV3 when intravesically administered to an immune-competent bladder cancer model. Using an established orthotopic bladder cancer model, 100 µL of 108 pfu mL−1 wild-type VSV, AV3, or UV-inactivated wild-type VSV (UVI) was instilled into the urinary bladders of female C3H mice. Half of the mice had normal, healthy bladders and were instilled with the oncolytic virus on day 0, 2, and 4. The remaining mice were given non-muscle invasive bladder tumors through the transurethral instillation of luciferase expressing MBT-2 mouse bladder carcinoma cells. On day 5 after initial tumor inoculation, tumor-bearing mice were imaged for bioluminescence and tumor size was quantified. On days 6, 8, and 10 after the initial tumor inoculation, tumor-bearing mice were randomized for treatment, and wild-type VSV, AV3, or UVI was instilled into each mouse. Levels of tumor bioluminescence (a correlate of tumor size), and body weight (a key variable of animal health and indicator of treatment toxicity) were monitored for 22 days. Following three consecutive intravesical treatments of an oncolytic virus, there were no differences in weight between the treated mice (wild-type VSV and AV3) and the control mice (UV-inactivated wild-type VSV), regardless of tumor presence. In addition, tumor bioluminescence in the AV3 treated mice was slightly decreased (30%) compared to the control mice, but not different from the wild-type VSV treated mice. These data correspond to in vitro data which show that wild-type VSV and AV3 are inhibitors of proliferation in MBT-2 mouse bladder carcinoma cells. In conclusion, our data strongly suggest that oncolytic viruses such as wild-type VSV and its attenuated form, AV3, can be used to treat superficial bladder cancer in immune-competent mice, without adversely affecting the health and well-being of the animal. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 2724. doi:10.1158/1538-7445.AM2011-2724
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.004 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".