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Evaluation of genetic diversity and relatedness among apple cultivars using RAPD and SSR markers

2016· article· en· W2323970755 on OpenAlexaboutno aff
Manju Modgil, Pardeep Pathani, Arjun Chauhan

Bibliographic record

VenueAgricultural Research Journal · 2016
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicPlant Physiology and Cultivation Studies
Canadian institutionsnot available
Fundersnot available
KeywordsRAPDCultivarGenetic diversityBiologyBiotechnologyDiversity (politics)Forensic scienceVeterinary medicineGeneticsBotanyHorticultureMedicineAnthropologySociology

Abstract

fetched live from OpenAlex

Twenty phenotypically different apple cultivars from the collection of Department of Fruit Science, YS Parmar University of Horticulture and Forestry, Solan, India, were analysed using 28 RAPD and 5 SSR primers to establish their genetic diversity, relatedness and verify parentage. The primer study revealed higher level of genetic diversity among the cultivars. The level of polymorphism across twenty cultivars was 65.6% and 71.4% from RAPD and SSR markers respectively. Primers OPA-16(RAPD), IISRS-3 and IISRS-18(SSR) were more informative due to the highest Polymorphism Information Content (PIC) value. Three RAPD primers were able to distinguish the cultivars. RAPD and SSR revealed different genetic similarities among apple cultivars. The highest similarity was detected between ‘Fuji’ and ‘Fuji Rekaki’ (0.859) followed by ‘Thanedar early flowering’ and ‘Fuji’ by RAPD while between ‘Galaxy’ and ‘Jonadel’ (1.000) followed by ‘Neomi’ with ‘Arlet’, ‘Fuji’ and ‘Royal Gala’; ‘Fuji Rekaki’ with ‘Thanedar early flowering’ and ‘Reinette-Du-Canada’ by SSR. The dendrograms from RAPD and SSR data were generated using UPGMA method which revealed four and five clusters respectively and showed that most of the cultivars clustered in accordance with the recorded pedigree information and polymorphism found with SSR primers was higher as compared to RAPD.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.002

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.147
GPT teacher head0.327
Teacher spread0.179 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations3
Published2016
Admission routes1
Has abstractyes

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