The neutral amino acid transporter B0 gene is up‐regulated in the jejunal villus cells compared to crypt cells measured by quantitative real‐time RT‐PCR in formula‐fed neonatal pigs
Bibliographic record
Abstract
The Na+‐neutral amino acid (AA) transporter B0 functions for the uptake of luminal neutral AA across the gut apical membrane. The aim of this study was to quantify B0 mRNA expression along the jejunal crypt‐villus axis in fed neonatal pigs. Six Yorkshire gilts were removed from sows at d 5 of age and fed a milk protein‐based liquid formula to 14‐16 d of age before tissue collection. Three epithelial cell fractions, representing cells from the upper villus, the middle villus and the crypt regions, were sequentially isolated, with cell viability of 92‐95% assessed by trypan blue exclusion, along the crypt‐villus axis from the entire jejunum by the distended sac method. The 443‐bp porcine partial mRNA sequence of the neutral AA transporter B0 gene was obtained according to the conserved region in the human gene sequence. The partial porcine mRNA sequence had 86 and 85% homology with the known human and mouse sequences, respectively. Real time RT‐PCR analyses (SmartCycler) using SYBR Green‐I detection kit revealed no differences (P>0.05) in the B0 mRNA abundance between the upper and the middle villus cell fractions but a lower B0 mRNA level (P<0.05) in the crypt cells by using β‐actin as the house‐keeping control. These results suggest that the B0 gene expression is low in crypt cells and is increased with cell differentiation in the villus cells in the formula‐fed neonate.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".