Role of the N-Terminal Domain of the Chaperone ClpX in the Recognition and Degradation of Lambda Phage Protein O
Bibliographic record
Abstract
The ClpXP ATPase-protease complex is a key element of the protein quality control machinery in the cell. ClpX consists of a zinc-binding domain (ZBD) that forms dimers and a AAA(+) domain that arranges into a hexamer in an ATP-dependent manner. Here, we report the binding site of the ClpX substrate λ phage protein O (λO) on ZBD(2) in ClpX using NMR and mutagenesis analysis. λO protein was found to interact with a hydrophobic patch on the larger surface of ZBD(2). The affinity of λO toward ZBD(2) was investigated using a quantitative optical biosensor method of dual polarization interferometry. The data suggest overlapping binding sites of λO and the ClpX cofactor SspB on the ZBD(2). Interestingly, a single key mutation in ZBD was found to enhance the ClpXP-dependent degradation of λO.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".