Inhibition of human pancreatic alpha-amylase by montbretin A: a new drug for diabetes and obesity?
Bibliographic record
Abstract
The gene encoding penicillin binding protein 4 and 5 of Haemophilus influenzae were cloned into the high-expression plasmid pET28 and overexpressed in Escherichia coli BL21 (DE3) star / pLysS respectively.Each proteins were purified more than 95 % and initial crystals were obtained by sitting-drop vapour-diffusion method at 293 K in a lot of conditions.After optimization of each conditions, the single crystals were grown at 293 K, 1 week for data collection.The HiPBP4 crystals belonged to space group P21 with unit-cell parameters a = 64.55Å, b = 92.59Å, c = 104.88Å and β = 107.75° .and the HiPBP5 crystals belonged to space group P212121 with unit-cell parameters a = 41.13Å, b = 53.01Å, c = 201.79Å.These crystals complete data set were collected at Photon factory.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".