Detection of Weak Absorption Changes from Molecular Events in Time-Resolved FT-IR Spectromicroscopy Measurements of Single Functional Cells
Bibliographic record
Abstract
The possibility of performing FT-IR spectromicroscopy experiments on individual living cells is the focus of considerable attention. Among the applications of interest, the obtainment of structural information in rapid measurements, with a time resolution of the minute or better, is a prized goal. In this work, we show that the use of synchrotron FT-IR spectromicroscopy allows one to extract weak spectral changes, of less than 10(-3) au per minute, in the absorption spectrum of single rod cells following photostimulation. We also show that absorption changes are accompanied by other optical effects due to changes in the real part of the refractive index of the cell. The use of two-dimensional correlation spectroscopy allows us to assign bands to specific molecular chromophores and to extract weak spectral variations in the presence of a noisy background.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".