Effect of cytochrome C on apoptosis of Hela cells induced by apoptin.
Bibliographic record
Abstract
Objective To study whether apoptin gene induces apoptosis of Hela cells via the release of cytochrome C from mitochondria and the activation caspase-8 and caspase-3.Method The Hela cells were transiently transfected with recombinant pcDNAA3 plasmid,which contained apoptin gene,and the apoptosis were measured with MTT methods. Caspase-8 and caspase-3 relative activity were analyzed by colorimetric assay at various time. The change of cytochrome C was monitored with Western blotting.Result The apoptin gene could induce apoptosis of Hela cells in vitro and the activity of caspase-3 began to rise in Hela cells after transfected with apoptin gene,but that of caspase-8 did not change. Western blotting showed more expression of cytochrome C in transfected Hela cells than that in control. Conclusion Apoptin gene can induce apoptosis in Hela cells via promoting the release of cytochrome C from mitochondria and then active caspase-3.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".