European and Asian Pears: Simple Sequence Repeat–Polyacrylamide Gel Electrophoresis-based Analysis of Commercially Important North American Cultivars
Bibliographic record
Abstract
The genus Pyrus (pear) includes species and cultivars of great diversity. We have tested the feasibility of a polyacrylamide gel eletrophoresis (PAGE)-based +/– simple sequence repeat (SSR) screen as a means of defining relationships amongst pears of commercial importance in North America. The screen included 28 pear accessions, including economically important cultivars, numbered selections from breeding programs and interspecific hybrids. It relied on 18 SSR primer pairs, each of which produced polymorphic banding patterns in all the genotypes examined. Fragments were scored for presence or absence within genotypes. The results show that amplification and analysis of a small number of SSR loci enable identification of cultivars and reasonable definition of genetic relationships in North American pears. Seven primer pairs were sufficient to distinguish the 28 pear cultivars. Analyses using both distance and parsimony criteria grouped cultivars in a manner consistent with known pedigrees and sites of origin.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.002 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".