Metabolism of uniformly labelled 13C‐eicosapentaenoic acid and 13C–arachidonic acid during healthy aging
Bibliographic record
Abstract
Long chain polyunsaturated fatty acids (LC‐PUFA) are involved in signalling pathways that control a wide range of bodily functions, notably inflammatory response. We previously gave a single dose of 13 C‐docosahexaenoic acid ( 13 C‐DHA) to six young (~25 yrs old) and six older (~75 yrs old) participants and we found that 4 h after the tracer intake, 13 C‐DHA was fourfold higher in the plasma of older participants than the young. Because previous studies reported that EPA (eicosapentaenoic acid) and AA (arachidonic acid) concentrations are age‐dependant, we sought to better characterize the kinetics of these two fatty acids between young and older participants. Objective To determine the kinetics of 13 C‐EPA and 13 C‐AA in young and older participants. Method Six young (18–30 yrs old) and six elderly (≥ 70 yrs old) healthy participants were recruited. The study is designed in three blocks of one month: 13 C‐EPA follow‐up, wash‐out and 13 C‐AA follow‐up. Each participant will orally consume a single oral dose of 35 mg of 13 C‐EPA, and after the wash out a 50 mg dose of 13 C‐AA. Fasted blood and breath samples will be collected before breakfast (baseline) and thereafter 2 h, 4 h, 6 h, 12 h, 24 h, 7 d, 14 d, 21 d and 28 d after the tracer intake. Breath samples will be collected in triplicate. 13 C enrichment in breath 13 CO 2 was measured by isotope ratio mass spectrometry. Preliminary results To date, two young and four elderly participants have started the study. Mean age of the young and the elderly is 24 ± 4 and 75 ± 2 yrs old, respectively. Weight and body mass index were not different between the two groups. At baseline, there was no difference in plasma triglycerides, HDL, LDL and total cholesterol between the two groups supporting that the elderly are metabolically healthy. Cumulative β‐oxidation of 13 C‐EPA over 7 days reached 40% in both groups. 13 C‐EPA and 13 C‐AA will be analyzed in the plasma of both groups. Aging is associated with low‐grade inflammation and EPA and AA are precursors of anti‐ and pro‐inflammatory molecules, respectively. Hence, this study will provide new insight into physiological modifications of these fatty acids metabolism associated with aging and could open new directions for research in this area. Support or Funding Information Quebec Network for Research on Aging. Pauline Léveillé is supported by a postdoctoral fellowship from the department of medicine, Faculty of Medicine and Health Sciences, University of Sherbrooke.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".