The actions of mouse mast cell protease-7 and calcium-activated potassium channel inhibitors on PAR2-induced relaxation of the aorta in mice
Bibliographic record
Abstract
Proteinase-activated receptor 2 (PAR2) may link inflammation with the progression of cardiovascular diseases. We examined the potential of mouse mast cell protease 7 (mMCP-7), an enzyme similar to human mast cell β-tryptase (hMCβ-tryptase), to activate PAR2-dependent vasodilation and the interaction of Ca²⁺-activated K⁺ channel (KCa ) activation by PAR2 with eNOS-dependent relaxation in the aorta. Aortas from mice expressing PAR2 (PAR2 +/+) and PAR2 knockout (PAR2 -/-) mice were used to bioassay the PAR2 activity of mMCP-7 and PAR2-activating peptide (2fly) in the presence of inhihitors of KCa.mMCP-7 did not produce relaxation responses like hMCβ-tryptase in the mouse aorta and inhihitors of KCa channels did not affect PAR2-mediated endothelial nitric oxide synthase dependent-relaxations of the aorta. These data highlight species and mechanistic heterogeneity in the ability of an orthologous enzyme agonist to activate PAR2 in different arteries. The results emphasize the need for cautions interpretations of studies assessing the pathophysiological role of PAR2 in different animal models.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.004 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".