Trichosantin differentially down regulates dendritic cells for their secretion of IL--12 and the expression of costimulation molecule CD80 in the high and low susceptible mouse strains
Bibliographic record
Abstract
Objective:To elucidate the mechanisms underlying the Trichosanthin (Tk)-induced immune suppression and its genetic control.Methods:Lymphoproliferation in vitro was adopted to measure the effects of Tk on dendritic cells for their ability of presenting antigen and activating T cells in high susceptible (HS) and low susceptible (LS) mouse strains. Bone marrow-derived immature dendritic cells (iBDCs) were treated with LPS (1 μg/ml) and recombinant mouse IFN-γ (20 ng/ml) for maturation. The concentration of IL-12 p40 in culture supernatants were detected by ELISA for the mature BDCs cultured with or without Tk. The expression of H-2 classⅡ, CD80, CD86, CD40 and CD11c molecules on BDCs were also determined by FACS.Results:For HS mouse strain (C57BL/6),the ability of BDCs for presenting and activating T cells was suppressed significantly in compared with a little suppression found in LS (C3H/He). The secretion of IL-12p40 was also decreased in C57B1/6 but not in C3H/He when the BDCs were cultured with Tk in LPS-containing medium. The expression of costimulatory molecule CD80 was down regulated for C57BL/6 and no change for C3H/He when Tk was added in the course of iBDC maturation. The expressions of other costimulation molecules we detected seemed not to be affected by Tk treatment both in HS and LS.Conclusion:Tk's ability of inducing suppression of T cell responses might depend on certain immunological condition of antigen-presenting cells, including their secretion of key cytokines and the expression of some costimulation molecules. All of them might be under the genetic control of MHC- related genes since there are quite differential patterns in responding to Tk stimulation for HS and LS mouse strains.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".