The effect of cleavage site of Newcastle disease virus F protein on the induction of autophagy in HepG2 cell line
Bibliographic record
Abstract
Newcastle disease virus(NDV) specifically elicits tumor apoptosis through the p53 independent innate pathway.The newest research indicated that NDV induced autophagy in U251 cell and resulting in cell apoptosis, but the underline mechanism remains unknown. In the present study, the recombinant NDV(rC30-FmF) was rescued by reverse genetics technology,which the cleavage site of the F protein of Clone 30 vaccine strain was replaced from to the GGRQGR↓L to the GRRQRR↓F of velogenic NDV. Then the HepG2 cell line was infected with the rC30-FmF and wild type Clone 30 strain(rC30-wt), respectively.Transmission electron microscopy examation shown that a large unmbers of autophagosomes and autolysosomes appeared in rC30-FmF infected HepG2 cells at 4 hours post infection. In addition, the expression of the autophagy marker protein(LC3II) was also significantly up-regulated in rC30-FmF infected cells, comparing with the rC30-wt infected cells(p0.01) detected by western blot. These results indicated that rC30-FmF was able to induce the formulation of autophagosomes in the early infection stage and the F protein cleavage play a key role in inducing autophagy in HepG2 cells.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".