Cloning and sequencing of piggyBac-like element in the Asian corn borer,Ostrinia furnacalis
Bibliographic record
Abstract
The piggyBac transposon which was identified from the cabbage looper Trichoplusia ni,is a highly useful transposon in insect genetic manipulation.To further understand the biological and evolutionary characteristics of piggyBac-like elements(PLEs)in insects,two PLE sequences,named as OfPLE1 and OfPLE2,were cloned from the Asian corn borer based on degenerate PCR,inverse PCR,RT-PCR and RACE.The sequence structure of OfPLE gene coding region and the phylogenetic relationship with other species were analyzed by application of the bioinformatics software.The results showed that OfPLE1 was 1 866 bp long encoding 607amino acid residues and OfPLE2 was 1 724 bp long encoding 434 amino acid residues.Furthermore,they shared 70% homology with each other,and had 32% identity and 26% identity with Trichoplusia ni piggyBac transposon.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".