Extraction of DNA and Optimization of RAPD-PCR Protocol in 11 Species of Cymbidium
Bibliographic record
Abstract
The method of CTAB-DNA isolation was optimized and used to extract genomic DNA from the tender leaves of 11 species of Cymbidium.DNA samples with superior quality and high yield could be gained by this method,and the A 260/A 280 values of tested samples were 1.7-1.9.To establish the reproducible RAPD-PCR protocol in Cymbidium,the concentrations of Mg2+,dNTPs,Taq DNA polymerase,and primers were also optimized in the present work.With the total volume of reaction was 25 μl,the optimal system using 75 ng genomic DNA,2.5 mmol/L Mg2+,0.15 mmol/L dNTPs,0.75 U Taq DNA polymerase,and 0.4 μmol/L primers,were employed to carry out PCR in 20 accessions from 11 species of Cymbidium.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".