Establishment of Proteomic Analysis of Suspension Cells in Betula platyphylla Suk.
Bibliographic record
Abstract
[Objective] To establish a two-dimensional polyacrylamide gel electrophoresis( 2-D PAGE) system for suspension cell proteomic analysis of Betula platyphylla. [Method]the improved acetic acid( TCA)-acetone precipitation method was applied for suspension cell protein isolation and purification,and the key technologies of electrophoresis including the sample loading quantity and types of IPG strip were optimized. [Reault]The results indicated that the reproducible 2-D gel with more spots and high resolution electrophoresis diagram could be obtained by using the improved TCA-acetone precipitation method,with 9. 0 μg / μl of loading protein sample on 17 cm IPG strip with p H 4-7. About 1 000 protein spots were obtained by PDQuest software analysis. 3 randomly selected protein spots were used to identify by matrix assisted laser desorption ionization time of flight mass spectrometry( MALDI TOF MS). The identified 3 proteins were S-S-adenosylmethionine synthetase,transposase for IS1330 and transcriptional regulatory proteins of PadR family,respectively. [Conclusion] The study laid the foundation for using proteomics analysis inducer to improve secondary metabolites accumulation mechanism.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".