Bioactive Change and Structural Change of Bacillus Amyloliquefaciens α-Amylases Induced by Ca~(2+)
Bibliographic record
Abstract
Based on the effect of bivalent calcium ions(Ca2 +) on the biological activity of Bacillus amyloliquefaciens α-amylases,the structural change of Bacillus amyloliquefaciensα-amylases induced by Ca2 +was studied via fluorescence spectroscopy and Fourier-transformation infrared spectroscopy. The results showed that when the concentration of Ca2 +in solution was below 25 mmol / L,the enzyme molecules could be activated by Ca2 +in solution,when the concentration of Ca2 +was over 25 mmol / L,the biological activity of enzyme molecules could be inhibited by Ca2 +. In the structural change of Bacillus amyloliquefaciens α-amylases induced by Ca2 +,only their secondary structures rather than their tertiary structures were involved; and when Ca2 +showed an activation effect to the bioactivity of the enzyme molecules,the contents of disorder structures andβ-sheet structures in the enzyme molecules decreased,and the α-helix ones and the β-turn ones increased.Whereas when Ca2 +showed an inhibition effect to the bioactivity of the enzyme molecules,theα-helix structures and β-turn structures in the enzyme molecules decreased,and disorder ones and theβ-sheet ones in the enzyme molecules increased.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".