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Record W23583773 · doi:10.1016/j.jmb.2013.04.005

Interleukin 1-beta Decreases Myoblast Fusion in vitro

2015· article· en· W23583773 on OpenAlexfundno aff
Bethany E Sullivan

Bibliographic record

Venue2014 ADSA-ASAS-CSAS Joint Annual Meeting · 2015
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMuscle Physiology and Disorders
Canadian institutionsnot available
FundersCanadian Institutes of Health Research
KeywordsBETA (programming language)In vitroChemistryComputer scienceBiochemistry

Abstract

fetched live from OpenAlex

During times of stress, systemic pro-inflammatory cytokine levels are increased, which may prevent optimal growth and development of muscle and/or induce muscle atrophy. Pro-inflammatory cytokines can induce negative responses in muscle by altering the balance of protein synthesis and degradation in established myofibers, or by influencing the proliferation and differentiation of myoblasts. Interleukin-1 beta (IL-1β) is a pro-inflammatory cytokine involved in stress and disease responses, but little is known about how IL-1β affects myoblast function. We hypothesized that IL-1β would decrease myoblast proliferation and/or differentiation. To test this hypothesis, C2C12 mouse myoblasts were treated with 0.1 ng/mL or 1.0 ng/mL of IL-1β, or carrier only (control). To determine proliferation rate, myoblasts were plated at 2.6x103 cells/cm2 and cultured for 48 h in the presence or absence of IL-1β. Cells were pulsed with bromodeoxyuridine (BrdU), fixed, and immunostained. The number of BrdU positive cells was quantified as a percent of total nuclei (identified by Hoescht 33342). To determine if IL-1β affected fusion, myoblasts were plated at 2.0x104 cells/cm2 in growth media for 48 h at which time media was changed into differentiation media supplemented with 0.1

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.004
Threshold uncertainty score0.013

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0040.002

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.016
GPT teacher head0.249
Teacher spread0.234 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2015
Admission routes1
Has abstractyes

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Same venue2014 ADSA-ASAS-CSAS Joint Annual MeetingSame topicMuscle Physiology and DisordersFrench-language works237,207