Optimization of Random Amplified Polymorphic DNA Reaction System in Phalaenopsis
Bibliographic record
Abstract
The modified SDS method was used for extraction DNA of Phalaenopsis,and reaction optimization of random amplified polymorphic based on progressive analysis of major factors to establish the best RAPD reaction system.The results showed that,concentration of DNA extracted reached 10 ng/μL,20 μL of optimized system was:0.5 μL of dNTPs(10 mmol/L),0.5 μL of DNA(10 ng/μL),2.4 μL of MgCl2(25 mmol/L),2 U of Taq polymerase,0.5 μL of primers(10 μmol/L),2 μL of 10×Buffer and 15.7 μL of ddH2O.Meanwhile,optimizing PCR procedure followed the 1 cycle of denaturing for 5 min at 94 ℃,42 cycles of denaturing for 1 min at 94 ℃,annealing for 1 min at 36 ℃,primer extension for 1.5 min at 72 ℃,and the last cycle of extension for 10 min at 72 ℃.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".