Bibliographic record
Abstract
An endo-glucanase can truncate long chains of cellulose in cellulose non-crystalline region through random hydrolysis of β- 1, 4- glycosidic linkage, which plays an important role in the overall degradation of cellulose. In order to improve the endo- glucanase activity of Aspergillus niger, a gene designated Eng1 encoding an endo-glucanase from Aspergillus niger CICC2462 was cloned by using the reverse transcription PCR method. Using the highly expressed elment of glaA encoding a glucoamylase, the recombinant expression vector pSZHG-Eng1 was constructed and transformed into the strain CICC2462 via the Agrobacterium-mediated method. Two strains were obtained by screening hygromycin resistant mutants and base pair substitution based on homologous recombination was established by PCR to exist in glaA gene loci gene. Under the shaking flask fermentation condition, the endo-glucanase activity of the A.niger transformant reached 272 U· mL- 1in the fermentation solution, which was 5.2-fold as high as that of the original strain. The approximately 36 ku protein band from two strains was observed on the SDS-PAGE, and the expressed enzyme was quantitated to be 165- 193 μg · mL- 1. This results showed that the endoglucanase gene was successfully transformed and homologously expressed in Aspergillus niger, which laid the foundation for the construction of an engineered strain to produce a food-grade endo-glucanase in large scale industrial production.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".