Preliminary Study on Proteomics Differentiation of Seed and Anther Between Cytoplasmic-Nuclear Male-Sterile Line W 931 A and Its Maintainer in Soybean
Bibliographic record
Abstract
Objective:The paper was aimed at the comparative proteome analysis of seed and anther between the cytoplasmic-nuclear male-sterile line W 931 A and its maintainer W 931 B in soybeans to obtain male-sterile mechanism.Method: the SDS-PAGE and LC-MS/MS.Result: The SDS-PAGE gel electrophoresis maps showed that no protein spots were found differentially expressed between the male-sterile line W 931 A and its maintainer W 931 B seeds,but 3 protein spots were found differentially expressed between W 931 A and W 931 B anthers at binucleate pollen stage.Conclusion:Above results inferred that expression of the male sterile genes was spacially and periodly,and the anther was the major organ in which the protein related to the male sterility was expressed.The function,especially those related to male sterility of the major differentially expressed proteins,including ADP-glucose pyrophosphorylase,NBS-LRR disease resistance protein homologue,ATP synthase,B subunit,thioredoxin-dependent peroxidase and zinc finger protein-like were reviewed and discussed.It was inferred that the male sterility of W 931 A might be related to starch synthesis,energy metabolism turbulence and cell death.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".