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Record W2361724475

Study on the Interaction of Bovine Serum Albumin with Riboflavin by Fluorescence Spectroscopy

2010· article· en· W2361724475 on OpenAlexaff
Min‐Yan Zheng

Bibliographic record

VenueJournal of Analytical Science · 2010
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicProtein Interaction Studies and Fluorescence Analysis
Canadian institutionsScience North
Fundersnot available
KeywordsChemistryQuenching (fluorescence)RiboflavinBovine serum albuminFluorescence spectroscopyFluorescenceSpectroscopyBinding constantPhotochemistryAnalytical Chemistry (journal)ChromatographyBinding siteBiochemistry
DOInot available

Abstract

fetched live from OpenAlex

The binding reaction of riboflavin with bovine serum albumin was studied by fluorescence spectroscopy.It was shown that riboflavin had a strong ability of quenching the fluorescence of BSA.The Stem-Volmer curve and double logarithm equation of the fluorescence quenching of BSA by riboflavin indicated that the quenching mechanism was a dynamic quenching;the binding constants(KSV) were 1.3658×105 L·mol-1(293 K) and 1.5082×105 L·mol-1(310 K),respectively.The thermodynamic parameters showed that the interaction of riboflavin and BSA was mainly driven by hydrophobic force.Synchronous spectroscopy was used to investigate the conformational changes of BSA.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.009
Threshold uncertainty score0.209

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.305
Teacher spread0.295 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2010
Admission routes1
Has abstractyes

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