Spectrophotometic Investigation on the Stable Conformations of Porcine Pepsin and Bovine Serum Albumin Induced by Denaturation Solutions
Bibliographic record
Abstract
The residual activities of porcine pepsin and bovine serum albumin induced by denaturant were studied with the UV spectroscopy or fluorescence spectroscopy.By means of the characteristic unfolding parameters,the distribution and transition of stable conformations of porcine pepsin and bovine serum albumin under different denaturant concentrations were well described.The results shows that during the three-states-unfolding of porcine pepsin induced by guanidine hydrochloride,the maximum(12%) intermediate content is obtained when the concentration of guanidine hydrochloride is 1.5mol/L;during the three-states-unfolding of bovine serum albumin induced by urea,the maximum(41%)intermediate content is obtained when the concentration of urea is 2.0 mol/L;during the four-states-unfolding of bovine serum albumin induced by guanidine hydrochloride,when the concentration of guanidine hydrochloride is approximately 0.4mol/L and 1.2mol/L,the maximum intermediate I1and I2content is achieved,accounting for 20%and 70%,respectively.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".